The purpose of this role is to generate genomic data that will enable research into the evolution of the Mycobacterium tuberculosis complex (MTBC) in Britain over the last five millennia in response to human behaviour and dramatic demographic changes, such as those caused by the Black Death. The MTBC comprises many lineages that cause TB in human and animal hosts, but only a handful of lineages are responsible for the high mortality and incidence observed globally today. Can the origin of these more "successful" lineages be traced to Britain, and did heightened periods of social inequality accelerate their adaptive evolution? The successful candidate will join the Wellcome Trust funded project, Exploring the contribution of social inequality to the evolution of the Mycobacterium tuberculosis complex across five millennia. To help answer the project's questions, they will perform all necessary lab work to prepare samples for Next Generation Sequencing, including DNA extraction, DNA quantification, DNA library preparation and indexing, library amplification and cleanup, and library quality control and pooling using Qubit, Tape Station, and qPCR technologies from hundreds of archaeological skeletal remains. They will also be responsible for performing in-solution hybridisation capture on promising libraries. Additionally, they will be responsible for preparing all the necessary buffers, solutions, and dilutions from stocks and performing routine operational checks and UV-sterilization of the restricted-access ancient DNA clean rooms.
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