Post-Doc : Genomic analysis on ancient human samples (Firenze)

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The aim of this research program is to analyse ancient Italian populations dated back from Palaeolithic to Medieval time, from a genomic point of view. In a first phase, the genetic material will be extracted from human skeletal remains through the most recent experimental protocols for the study of degraded DNA and a Next Generation Sequencing approach for producing genomic data. After a first evaluation of the endogenous DNA content through an explorative shotgun sequencing of the NGS libraries, a deep sequencing will be programmed after a possible Whole Genome Enrichment step on samples with lower endogenous DNA percentages. Then, sequence data will be analysed with bioinformatics tools in order to reconstruct the genetic profiles after authentication according to the ancient DNA analysis criteria (i.e. EAGER, ANGDS). The genetic profiles will be analysed at individual and population level. With a focus on each single necropolis, the phenotypic traits of individuals will be estimated (physical appearance, metabolic traits, diseases) and possible kinship relationships will be reconstructed with software for ancient DNA data (i.e. READ, NgsRelate). Population genetics analysis will be performed, to describe genetic variability at intra- and inter-population level, estimating genomic similarity between populations (Fst, PCA, f-statistics, admixture) and possible relationships (migrations and genetic flux). The obtained information will be compared to other evidences from different research fields such as archaeology.

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