One PhD position is available at the University of Bordeaux starting September 1st, 2020 as a part of the European Training Network PUSHH Palaeoproteomics to Unleash Studies on Human History funded by the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement No 861389. This vacancy offers a three year, fully funded PhD on Mass Spectrometry for Palaeoproteomics (reference ESR10). You will be supervised by Prof Caroline Tokarski with cosupervision by Prof Jane Thomas-Oates.
The latest generation of high-resolution tandem mass spectrometry for palaeoproteomics can reliably and rapidly detect proteins in complex mixtures, even in the very limited quantities typical of ancient samples. Workflow miniaturization and simplification of sample preparation, by integrating into a single or few step(s) different chemical treatments, have already been proven to improve recovery in the most recalcitrant ancient samples. Such approaches are contributing to minimizing the amount of starting material needed for palaeoproteomics analysis. The possibility to combine the dissociative capabilities of complementary fragmentation methods (ETD, UVPD, HCD) maximises the ability to elucidate the full complexity of the protein material, in sequence, in polymorphism and in chemical and/or post-translational modifications with unprecedented depth and accuracy. Top-down methods, adding molecular details lost when proteoforms are hydrolyzed into proteolytic peptides in bottom-up proteomics, also benefit from the recent technological gains in mass spectrometry. The ESR10 will develop new minimally invasive methods and miniaturized analytical workflow based on bottom-up and top-down approaches, to analyse trace level of proteins in palaeoanthropological and palaeontological materials and reach an exhaustive characterization of ancient material available.
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